De Novo Design Of Bioactive Protein Switches. Mutagenic primers were designed to anneal >18 bp on eit

Mutagenic primers were designed to anneal >18 bp on either side of the site for mutagenesis, with the desired mutation en The ability to design switchable protein functions that are controlled by induced conformational change is a milestone for de novo protein design, and opens up new avenues for synthetic biology and cell There has been considerable progress in the de novo design of stable protein structures based on the principle that proteins fold to their lowest free energy state. We explore the Allosteric regulation of protein function is widespread in biology, but is challenging for de novo protein design as it requires the explicit design of multiple states with comparable free energies. We design a static, five-helix 'cage' with a single We describe orthogonal Cage-Key systems that function in vitro, in yeast and in mammalian cells with up to 40-fold activation of function by Key. Controlled localized conformational changes, common in natural switches, have not yet been Here we explore the possibility of designing switchable protein systems de novo, through the modulation of competing inter- and intramolecular Allosteric regulation of protein function is widespread in biology, but challenging for de novo protein design as it requires explicit design of multiple states with The design of tunable and generalizable protein switches is a considerable advance for de novo protein design. In the first class, protein-protein Abstract: Abstract Allosteric regulation of protein function is widespread in biology, but is challenging for de novo protein design as it requires the explicit design of multiple states with comparable free De Novo Design of Bioactive Protein Switches, and Applications Thereof Robert Andrew Langan Chair of the Supervisory Committee: David Baker Department of Biochemistry Allosteric regulation of De novo design of bioactive protein switches. Allosteric regulation of protein function is widespread in biology, but is challenging for de novo protein design as it requires the explicit design of multiple states The design of switchable protein function controlled by induced conformational change is a milestone for de novo protein design and opens up new avenues for synthetic biology and cellular engineering. We explore the Here we explore the possibility of designing switchable protein systems de novo, through the modulation of competing inter- and intramolecular interactions. Langan等发表于Nature的论文De novo design of bioactive protein switches介绍了一种完全从头设计的 生物活性蛋白 开关，设计 Allosteric regulation of protein function is widespread in biology, but is challenging for de novo protein design as it requires the explicit design of multiple states with comparable free energies. In the switchable LOCKR system described here, a designed key added in trans induces a Addgene is a nonprofit plasmid repository. 2019年7月24日，由Robert A. All primers for mutagenesis were ordered from Integrated DNA Technologies (IDT). Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, El Allosteric regulation of protein function is widespread in biology, but challenging for de novo protein design as it requires explicit design of multiple states with comparable free energies. 3 LOCKR蛋白示意图 开关系统依赖于LOCKR（Latching Orthogonal Cage–Key pRotein）技术，两篇论文共同发表在7月24日的Nature杂 DegronLOCKR designer-protein technology is used to implement synthetic positive- and negative-feedback systems in the yeast Here we describe the de novo design of two classes of protein switches which link phosphorylation by tyrosine and serine kinases to protein-protein association. These de novo -designed proteins hold great promise as building blocks for synthetic circuits and being able to control protein function in Allosteric regulation of protein function is widespread in biology, but challenging for de novo protein design as it requires explicit design of multiple states with comparable free energies. Here we explore the possibility of designing switchable protein systems de novo, through the modulation of competing inter- and intramolecular Here we explore the possibility of designing switchable protein systems de novo, through the modulation of competing inter- and intramolecular interactions. The design of switchable protein function controlled by The ability to design switchable protein functions that are controlled by induced conformational change is a milestone for de novo protein design, and opens up new avenues for synthetic biology and cell De novo design of bioactive protein switches. Allosteric regulation of protein function is widespread in biology, but is challenging for de novo protein design as it requires the . Protein switches are essential in biology and important targets for protein design.

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